Journal: Journal of Diabetes Investigation

Article Title: Liraglutide combined with dapagliflozin treatment improves myocardial disease and endothelial dysfunction in T2DM mice

doi: 10.1111/jdi.70363

Figure Lengend Snippet: Combination therapy enhances endothelial integrity and activates NRF2/HO‐1 and AMPK/PKA‐eNOS pathways to counteract vascular oxidative stress. (a) Immunofluorescence staining of aortic sections for CD31 (endothelial marker, red) and 3‐nitrotyrosine (3‐NT, oxidative damage marker, green); nuclei stained with DAPI (blue). Scale bar: 50 μm. (b) Quantification of CD31 + area (%) and 3‐NT fluorescence intensity ( n = 6 per group). (c) Western blot analysis of NRF2, HO‐1, phospho‐AMPK (Thr172), total AMPK, PKA substrate phosphorylation, eNOS, and phospho‐eNOS (Ser1177) in aortic tissue. β‐Actin served as loading control, n = 3. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01 vs. control; ## P < 0.01 vs. model.

Article Snippet: Membranes were blocked with 5% (w/v) non‐fat milk (A600669‐0250, Sangon Biotech) in TBST for 1.5 h at room temperature and then incubated overnight at 4°C with the following primary antibodies at specified dilutions: TNF‐α (1:1,000, 17590‐1‐AP, Proteintech), IL‐1β (1:1,000, AF5103, Affinity), IL‐6 (1:500, GB11117, Servicebio), Bax (1:8,000, 50599‐2‐Ig, Proteintech), BCl‐2 (1:1,000, AF6139, Affinity), cleaved caspase‐3 (1:1,000, AF7022, Affinity), 3‐NT (1:1,000, HY‐ P81216 , MCE), NRF2 (1:1,000, BF8017, Affinity), HO‐1 (1:20,000, 10,701‐1‐AP, Proteintech), p‐AMPK (1:1,000, AF3423, Affinity), AMPK (1:1,000, AF6423, Affinity), PKA (1:1,000, AF7746, Affinity), p‐eNOS (1:1,000, AF3247, Affinity), and eNOS (1:20,000, 27120‐1‐AP, Proteintech).

Techniques: Immunofluorescence, Staining, Marker, Fluorescence, Western Blot, Phospho-proteomics, Control

Journal: Current Therapeutic Research, Clinical and Experimental

Article Title: The Berberine Derivative BBR684 Inhibits VDAC Oligomerization to Suppress Ferroptosis in Acute Kidney Injury

doi: 10.1016/j.curtheres.2026.100825

Figure Lengend Snippet: BBR684 attenuates Erastin-induced ferroptosis in HK-2 cells by suppressing lipid peroxidation and activating the antioxidant response. (A) Flow cytometric analysis of lipid peroxidation using the C11-BODIPY probe in HK-2 cells treated as indicated for 12 hours. Data are presented as mean fluorescence intensity (MFI). (B) Intracellular Fe²⁺ levels measured by a ferrous iron colorimetric assay. (C, D) Levels of malondialdehyde (MDA, C) and reduced glutathione (GSH, D) in cell lysates. (E, F) Representative immunofluorescence images (E) and quantification (F) of 4-hydroxynonenal (4-HNE) adducts (red) in HK-2 cells. Nuclei were counterstained with DAPI (blue). Scale bar, 50 µm. (G) Western blot analysis of key ferroptosis-related proteins: glutathione peroxidase 4 (GPX4), heme oxygenase-1 (HO-1), and nuclear factor erythroid 2–related factor 2 (NRF2). β-actin served as the loading control. Data are from 3 independent biological replicates (n = 3) and presented as mean ± SEM. Significance was determined by one-way ANOVA followed by Tukey's HSD post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001; n.s., not significant.

Article Snippet: Antibodies against GPX4 (Catalog #3F5G5), HO-1 (Catalog #10701-1-AP), NRF2 (Catalog #16396-1-AP), VDAC (Catalog #10866-1-AP), Cleaved Caspase-3 (Catalog #68773-1-Ig), and CD45 (Catalog #98035-1-RR) were purchased from Proteintech.

Techniques: Fluorescence, Colorimetric Assay, Immunofluorescence, Western Blot, Control